Toyooka Lab


Home for Neurodevelopment

IL-12 produced by antigen-presenting cells induces IL-2-independent proliferation of T helper cell clones.


Journal article


S. Maruo, K. Toyo-oka, M. Oh‐hora, X. Tai, H. Iwata, H. Takenaka, S. Yamada, S. Ono, T. Hamaoka, M. Kobayashi, M. Wysocka, G. Trinchieri, H. Fujiwara
Journal of immunology, 1996

Semantic Scholar PubMed
Cite

Cite

APA
Maruo, S., Toyo-oka, K., Oh‐hora, M., Tai, X., Iwata, H., Takenaka, H., … Fujiwara, H. (1996). IL-12 produced by antigen-presenting cells induces IL-2-independent proliferation of T helper cell clones. Journal of Immunology.

Chicago/Turabian
Maruo, S., K. Toyo-oka, M. Oh‐hora, X. Tai, H. Iwata, H. Takenaka, S. Yamada, et al. “IL-12 Produced by Antigen-Presenting Cells Induces IL-2-Independent Proliferation of T Helper Cell Clones.” Journal of immunology (1996).

MLA
Maruo, S., et al. “IL-12 Produced by Antigen-Presenting Cells Induces IL-2-Independent Proliferation of T Helper Cell Clones.” Journal of Immunology, 1996.


Abstract

We investigated the role of IL-12 in proliferation of various Th cell clones (class II-alloreactive (4-86 and 4-55) and keyhole limpet hemocyanin + self I-Ek-reactive (9-16)) following stimulation with Ag on APCs. These clones proliferated in response to stimulation with rIL-2, rIL-12, or Ag/APC. The proliferation induced by Ag/APC stimulation was not affected by anti-IL-2 Ab but was markedly inhibited by anti-IL-12 Abs. Consistent with this finding was the absence of detectable IL-2 activity in culture supernatants 12 to 48 h after Ag/APC stimulation, and the detection of significant levels of IL-12 in an Ab-capture bioassay. IL-12 was produced within 12 h after Ag/APC stimulation, reaching a peak after 18 to 24 h. The production of IL-12 in cultures of Th clones and APC contrasted with the production of IL-2 but not IL-12 upon allostimulation of primary T cells and the inhibition of their proliferation exclusively by anti-IL-2 Abs. Analysis of the expression of IL-12-binding sites on Th cells revealed low levels of IL-12 receptors in resting Th clones but high IL-12R levels 2 to 3 days after Ag/APC stimulation, declining gradually thereafter. The changes in IL-12R expression levels correlated closely with the IL-12 responsiveness of Th populations at various times after Ag/APC stimulation; Th populations obtained 3 and 10 days after Ag/APC stimulation exhibited very high and weak or marginal responsiveness to rIL-12, respectively, whereas the responses to rIL-2 were comparable in both Th populations. These results indicate that the Ag/APC-stimulated proliferation of terminally differentiated Th clones, in contrast to naive T cells, depends on the production of IL-12 by APC and on the simultaneous up-regulation of IL-12R on Th cells rather than on an IL-2 autocrine mechanism.


Share



Follow this website


You need to create an Owlstown account to follow this website.


Sign up

Already an Owlstown member?

Log in